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Kisspeptin treatment in mesenchymal bone cells induces markers of inflammation Publique Deposited

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MLA citation style

Vroegop, Joe, et al. Kisspeptin Treatment In Mesenchymal Bone Cells Induces Markers of Inflammation. . 2021. https://mushare.marian.edu/concern/generic_works/67e2b2da-739b-4793-8512-8e2d1cc3e742?locale=fr

APA citation style

Vroegop, Joe, Matz, Jordan, Kruse, Kasey, Fahrholz, Chynna, Lowery, Jonathan, Castanon, Mariah, Lemmon, Shelby, & Hum, Julia. (2021). Kisspeptin treatment in mesenchymal bone cells induces markers of inflammation. https://mushare.marian.edu/concern/generic_works/67e2b2da-739b-4793-8512-8e2d1cc3e742?locale=fr

Chicago citation style

Vroegop, Joe, Matz, Jordan , Kruse, Kasey, Fahrholz, Chynna , Lowery, Jonathan , Castanon, Mariah , Lemmon, Shelby et al. Kisspeptin Treatment In Mesenchymal Bone Cells Induces Markers of Inflammation. 2021. https://mushare.marian.edu/concern/generic_works/67e2b2da-739b-4793-8512-8e2d1cc3e742?locale=fr

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Kisspeptin together with its receptor, GPR54 (Kiss1R), are known for their function as the principal regulators of the onset of mammalian puberty. Given the characteristic growth spurt and bone development that occurs during puberty, recent studies have provided insights into the role kisspeptin has in the control of skeletal homeostasis as well as bone cell differentiation. Both kisspeptin and Kiss1R knockout ice display decreased trabecular bone mass. The present study hypothesizes kisspeptin communicates with bone cells which may aid to regulate growth and differentiation. The objective of this work was to establish the expression patterns of kisspeptin and Kiss1R in bone and uncover for the cell signaling networks it can affect. Mesenchymal bone cells, W-20-17 (W20), were analyzed via western blot analysis and found to express Kiss1R. W20s were then treated with the kisspeptin peptide (50µM) for approximately 18 hours in starving media. Signal transduction antibody microarrays were run to compare changes in the intracellular signaling networks affected by kisspeptin treatment (n=3). Of the 165 specific antibodies targets related to signaling cascades, 23 targets were significantly increased or decreased (p<0.05). Interleukin-5 (IL-5) and Tumor necrosis factor-α (TNF-α) expression was significantly increased by kisspeptin treatment (1.86 and 1.24 respective fold, p<0.05). Work is ongoing to identify if this response is Kiss1Rdependent. These data suggest kisspeptin treatment can induce an inflammatory response in bone.

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