Document Type

Article

Publication Date

7-30-2019

Published In / Presented At

Capstone Final Project

Disciplines

Biological Phenomena, Cell Phenomena, and Immunity | Medical Cell Biology | Medical Microbiology

Abstract

This experiment analyzes three different techniques used in literature to detect cytokine release from cells in vitro: the Human Cytokine Array (HCA) by R&D Systems, the BD Cytometric Bead Array (CBA) assay, and protein enzyme-linked immunosorbent assay (ELISA). The model used to test these assays was a human dermal fibroblast cell culture that was subjected to strain profiles by the Flexcell FX-6000 that include a repetitive motion injury, a profile with the injury strain as well as a strain representing myofascial release, and a control that was not strained. Each of these techniques analyzed the conditioned media of the cultures collected 96 hours after the strain profile was completed. The HCA was proficient at analyzing a large amount of cytokines at once, though was less sensitive than the other assays. The CBA measured six cytokines at once and showed high sensitivity in detecting differing concentrations in the conditioned media. The ELISA could only measure one cytokine at a time, but also showed high sensitivity in detecting differing concentrations in the conditioned media. This experiment also looked at the cost and amount of sample used. This experiment also considers price, amount of sample used and duration of the assay to determine ideal situations to use each technique.

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