Medicine and Health Sciences
Probiotics are known beneficial or commensal microorganisms. They are often recommended as alternative treatments for Clostridium difficile (C diff) infections. It is theorized that supplementation with probiotic organisms competes against established toxin producing C diff organisms. The aim of this study is to compare the inhibition of C diff by various commercial probiotics including Florastor®, Align®, and Nexabiotic® that contain various potencies and numbers of probiotic strains. Design: This will be an in vitro study performed at Marian University. Methods: C diff bacterium will be grown anaerobically and then mixed with dilutions of over the counter probiotic organisms as well as a saline control. The mixtures will be incubated anaerobically for a period of 48 hours and then analyzed via real time quantitative PCR. Cycle threshold numbers for the various mixtures will then be compared to determine the level of growth inhibition. Results: This study will be conducted in the near future. Previous studies have concluded that probiotic products containing multiple organisms may have greater inhibitory effects (PMC3354390) although specific name brand probiotics have not been tested.
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Tran, Bryan A.; Doa, Ella; and Larsen, Bryan Ph.D., "Quantitative Real Time PCR Detection of In Vitro Clostridium Difficile Growth Inhibition by Popular Over-the-Counter Probiotic Supplements" (2015). MU-COM Research Day. 5.