Introduction: Kisspeptin together with its receptor, GPR54 (Kiss1 R), are known for their function as the principal regulators of the onset of mammalian puberty. Given the characteristic growth spurt and bone development that occurs during puberty, recent studies have provided insights into the role kisspeptin has in the control of skeletal homeostasis as well as bone cell differentiation. Objective: The present study hypothesizes kisspeptin and/or its receptor are expressed by bone cells and provide a communication pathway to regulate growth and differentiation. The objective of this work is to establish the expression patterns of kisspeptin and Kiss1R across various bone cell lines, as well as changes in their respective expression patterns with aging and mechanical stress. Methods: Osteoblast precursor cell line, MC3T3- E1, was cultured and cells were harvested to perform western blots to characterize the baseline expression patterns of kisspeptin and Kiss1R, respectively. Ongoing studies: Next, the role of kisspeptin and its receptor will be investigated in MC3T3-E1 cells placed under mechanical stress, via an orbital shaker. In addition, the expression pattern of kisspeptin and its receptor will be characterized in the long bones of young and aged mice. Future studies: Examine the utility of pharmacologically targeting a kisspeptin signaling pathway to promote anabolic bone growth.

Creator

• Hum, Julia

• Matz, Jordan

Language

• English

Palabra Clave

• Kisspeptin

• Bone Cell Differentiation & Growth

• skeletal homeostasis

Date created

• 2020

Related URL

• Audio presentation:  https://marian.hosted.panopto.com/Panopto/Pages/Viewer.aspx?id=16b23e0b-1fb0-47c4-801c-ac74003f2eb4

Resource type

• Poster

Rights statement

• http://rightsstatements.org/vocab/InC/1.0/