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Neuromedin-U (NMU) negatively regulates bone remodeling

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MLA citation style (9th ed.)

Zubosky, Tara, et al. Neuromedin-u (nmu) Negatively Regulates Bone Remodeling. . 1122. mushare.marian.edu/concern/generic_works/0cca2bb9-658f-4595-a14f-6788d079a3d0?locale=zh.

APA citation style (7th ed.)

Z. Tara, J. Kelli, H. Yu-tin, J. Krista, L. Jonathan, & S. Maria. (1122). Neuromedin-U (NMU) negatively regulates bone remodeling. https://mushare.marian.edu/concern/generic_works/0cca2bb9-658f-4595-a14f-6788d079a3d0?locale=zh

Chicago citation style (CMOS 17, author-date)

Zubosky, Tara, Jestes, Kelli, Hsiao, Yu-Tin, Jackson, Krista, Lowery, Jonathan, and Squire, Maria. Neuromedin-U (nmu) Negatively Regulates Bone Remodeling. 1122. https://mushare.marian.edu/concern/generic_works/0cca2bb9-658f-4595-a14f-6788d079a3d0?locale=zh.

Note: These citations are programmatically generated and may be incomplete.

300 targets using very small quantities of protein. This revealed decreased activation of the mTOR pathway in tibiae of Nmu knock-out mice compared to controls, with reduced activity-associated phosphorylation of mTOR and the mTOR target ribosomal protein S6. To begin establishing the skeletal NMU pathway, we determined that both Nmur1 and Nmur2 are expressed in bone and osteogenic cells. Cell-based in vitro assays indicate that NMU-25 reduces osteoblastic differentiation of W-20-17 murine bone marrow stromal cells (BMSCs) whereas NMU-8 has no effect on osteoblastic differentiation. In contrast, NMU-8 treatment of differenti-ated W-20-17 cells leads to increased proliferation whereas NMU-25 does not. Consistent with this, NMU-8 treatment of the osteoblast-like cell line MC3T3-E1 leads to increased proliferation and expression of alkaline phosphatase. Phospho-profiling ar-rays are currently underway to examine the mechanism(s) allowing for differential effects between NMU-8 and NMU-25 and the response of undifferentiated BMSCs versus osteoblastic cells. Collectively, our work highlights a novel factor associated with bone remodeling and may identify a new opportunity for treating low bone density in humans.

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