... worldwide problem with enormous ecological and economic consequences [19] [20]. Though many of these non ...
... from two seedlings in 250 mL of sterile TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) for 20 - 25 min at ...
... represent each of 20 composite rhizosphere soil samples. Clones were grown overnight in LB Broth (Thermo ...
... Fisher Scientific, USA) with ampicillin (100 gmL1) and stored at 80C in 20% glycerol. Methods for plasmid ...
... representation followed by -, -, and -Proteobacteria except in CS2018, when -Proteobacteria had 20 clones vs 16 ...