Cutaneous wound healing is an intricate and multifaceted process. Despite these complexities, the distinct phases of wound healing provide a unique opportunity to evaluate the roles of different targets in these coordinated responses. This protocol details an in vivo wound healing assay to study the intersection of cellular, molecular, and systemic effector pathways. The role of certain proteins in the wound healing process can be efficiently explored in vivo through the generation of tissue-specific deficient mice. This approach, although optimized for use with animal models displaying epithelial deficiencies, can be used for other tissue-specific deficiencies, and utilizes simple and cost-effective methods, allowing investigators to precisely devise their experimental design. The coordination of immunological, epithelial, vascular, and microenvironmental factors in wound healing makes this technique a valuable tool for investigators across fields.
Raised just outside Cincinnati, in the rural community of Rossmoyne, Ohio, Aaron Belford Thompson, Priscilla Jane Thompson, and Clara Ann Thompson wrote poetry that addressed Black American life in the early twentieth century. In their time, they were hailed as literary celebrities in the Cincinnati and Indianapolis Black communities, but they never managed to attain mainstream attention. This book presents three of their poetry collections-Aaron's Harvest of Thoughts, Priscilla's Gleanings of Quiet Hours, and Clara's Songs from the Wayside, all initially published in 1907-1908-as a snapshot into the artistic and political interests of this talented family of poets.