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10/23/1959 0:00
Cancella il filtro <p>10/23/1959 0:00</p> CreatoreDoci, Colleen L.
Cancella il filtro Creatore: <p>Doci, Colleen L.</p>Risultati della ricerca
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- ... STEM Issues Global Issues Career Issues 0 2 4 6 8 10 12 IDENTITY AND INTERSECTIONALITY STEM Identity is ...... Minority barriers Senior Seminar Format Rankings 0 1 2 3 4 Group Discussion/ In-Class Activities 5 Markers ...
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Numerous obstacles contribute to the underrepresentation of racial, ethnic, and gender groups in science and technology fields. However, addressing these barriers is complex and multifactorial. As part of their exploration of...
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- ... . NOL7 mRNA and protein levels were assessed in 13 CC cell lines and 23 consecutive CC specimens by real ...... media were supplemented with 10% FBS, penicillin (100 mg/mL), and streptomycin. Media were as follows ...... Amplication Genomic DNA from the cell lines, normal cervical mucosa, and 23 CC samples were treated with ...... 10 minutes, 35 cycles of 941C for 30s, 591C for 45s, 721C for 45s, and 721C for 10 minutes. The PCR ...... for 30 seconds, 721C for 2 minutes, and 721C for 10 minutes. PCR products were puried using the Wizard ...
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Doci, Colleen L., Langerman, Alexander, Kanteti, Rajani, Lingen,Mark W., Ostler, Kelly R., Onel,Kenan, Godley, Lucy A., Mankame, Tanmayi P., Best, Timothy, and Salgia, Ravi
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NOL7 is a putative tumor suppressor gene localized to 6p23, a region with frequent loss of heterozygosity in a number of cancers, including cervical cancer (CC). We have previously demonstrated that reintroduction of NOL7 into...
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- ... 66 76-100 51-75 26-50 1-25 80 60 40 20 0 PD 30 10 1 3 0.3 Recombinant DSG3 (ng) 100 Tubulin PD DSG3 ...... DSG3 (fg mL-1) 40 750 500 250 30 DSG3 PDDA HRP Ab2 I, nA 100 AuNPs 50 20 25 5 10 10 0 0 1 m ...... streptavidin coated magnetic bead -10 0 400 800 1200 1600 t, s Ab2 -MB-HRP 40 35 y = -7.4659 + 14.987log(x) R ...... = 0.99315 30 I, nA 25 20 15 10 5 Voltage + H2O2 HQ amperometric signal 0 100 10 C 1000 [DSG3], pg mL-1 1000 ...... 26 April 2012 Received in revised form 19 July 2012 Accepted 1 August 2012 Available online 23 ...
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Singh, Bhuvanesh, Marsh, Christina A., Sinha,Uttam K., Rusling, James F., Malhotra, Ruchika, Veenstra, Timothy D., Doci, Colleen L., Gutkind, J. Silvio, Patel, Vyomesh, Nathan, Cherie-Ann O., Martin, Daniel, and Molinolo, Alfredo A.
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OBJECTIVES: The diagnosis of cervical lymph node metastasis in head and neck squamous cell carcinoma (HNSCC) patients constitutes an essential requirement for clinical staging and treatment selection. However, clinical...
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- ... factor (VEGF)10 and sphingosine-1-phosphate (S1P)11,12. Global deficiency of each Rho GTPase leads to ...... embryonic lethality by E9-10 due to deficiencies in lumen formation and failed blood circulation. Inducible ...... complete medium). Scientific Reports | (2019) 9:11666 | https://doi.org/10.1038/s41598-019-48053-z 10 ...... was performed as described previously73. The cells were lysed on ice in RIPA buffer (10 mmol/L Tris ...... Inhibitor Cocktail; Thermo Scientific). Cell lysates were centrifuged at 13,000 rpm for 10 min at 4 C and ...
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Tuz Zahra, Fatema, Kubota, Yoshiaki, Doci, Colleen L., Minchew, Shelby A., Cobos, Christopher, Gutkind, J. Silvio, Mikelis, Constantinos M., Zheng, Yi, Ichiyama, Yusuke, Sajib, Sanaullah, Tullar, Paul E., and Akwii, Racheal Grace
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Imbalanced angiogenesis is a characteristic of several diseases. Rho GTPases regulate multiple cellular processes, such as cytoskeletal rearrangement, cell movement, microtubule dynamics, signal transduction and gene...
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- ... starved for 6 hours and then treated for 3 hours with either 0, 1, or 10 ng/ml TGF-1. Cells were fixed ...... ]. Deletion of Gnaq alone results in ataxia, impaired motor control, and skin pigmentation [10, 11, 14, 23 ...... transfected, reconstituted expression of Gq were allowed to migrate towards 0, 1, or 10 ng/ml TGF-1. C ...... minutes treatment with 0, 1, or 10 ng/ml TGF-1. D) G11/q-null and reconstituted cells co-expressing ...... LifeActGFP were treated with 0, 1, or 10 ng/ml TGF-1 for 3 hours and assessed for cytoskeletal changes ...
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Mikelis, Constantinos M., Gutkind, J. Slivio , Molinolo, Alfredo A. , Inoue, Asuka , Callejas-Valera, Juan Luis , Doci, Colleen L., Hansen, Karina K. , and Offermanns, Stefan
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G-protein coupled receptors (GPCRs), which activate heterotrimeric G proteins, are an essential class of transmembrane receptors that are responsible for a myriad of signaling events in normal and pathologic conditions. Two...
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- ... beyond 5 years (9, 10). This is partially attributed to the propensity of HNSCC to metastasize to ...... methods. Cell culture 293T-17, HaCat, COS-7, UMSCC2, and UMSCC17B cells were cultured in DMEM 10% FBS ...... the intensity and the percentage of positive cells quantied as described (23). Correlations were ...... xenografts in SCID/NOD mice All animal studies were carried out according to NIHapproved protocols (ASP# 10 ...... cancers cases. Tissues were evaluated for intensity and percentage of positive cells, with 0 being absent ...
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Mikelis, Constantinos M., Doci, Colleen L., Gutkind, J. Silvio, Lionakis, Michail S., and Molinolo, Alfredo A.
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Head and neck squamous cell carcinomas (HNSCC) often metastasize to locoregional lymph nodes, and lymph node involvement represents one of the most important prognostic factors of poor clinical outcome. HNSCCs are remarkably...
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- ... . Putative Sequence (PS) 1, amino acids 1-10 appeared to be a monopartite NLS, while PS2, amino acids 88-112 ...... all four PSs [1-10, 88-257] was cytoplasmic (Figure 3B). Further, PS1 and PS2 were shown to function ...... individually as NLSs, as constructs missing PS2, PS3, and PS4 [88-257] or PS1, PS3, and PS4 [1-10, 113-257 ...... nucleoplasmic mutant N23(-), was least efficiently targeted. The single mutants demonstrated nearly 10% more ...... NLS2 and NLS3, with over a 15% decrease in efficiency for N23(-) compared to WT NOL7 and over 10 ...
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BACKGROUND: NOL7 is a candidate tumor suppressor that localizes to a chromosomal region 6p23. This locus is frequently lost in a number of malignancies, and consistent loss of NOL7 through loss of heterozygosity and decreased...
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- ... -test from four independent experiments. *Po1 10 5; **Po1 10 8; n.s., not significant. (d) SiHa ...... 10 6; ** Po1 10 10; n.s., not significant. Oncogene (2013) 4377 4386 & 2013 Macmillan ...... . Proteins associated with poly(A) mRNA were pulled down using oligo(dT) cellulose. Input lanes represent 10 ...... -test relative to NOL7 input (a, b) and relative to the NOL7-bound (c, d) fractions. aPo0.002; bPo4 10 ...... ). *Po0.001, **Po1 10 4. Data are represented as means.e.m. from three independent assays. NOL7 post ...
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Thrombospondin-1 (TSP-1) is an endogenous inhibitor of angiogenesis whose expression suppresses tumor growth in vivo. Like many angiogenesis-related genes, TSP-1 expression is tightly controlled by various mechanisms, but there...
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- ... Vehicle CXCL12 500m 3 I 7 *** 50 ventral tongue 60 Time (days) 0 100 80 0 10 150 100 0 1 shControl shCXCR4 ...... . After selection of cell pools resistant to puromycin, we 1061 0 10 0 10 1 10 2 10 3 Figure 3. Loss-of ...... most of the animals (16 of 20) free of metastases at the end of the 1063 0 10 0 0 10 0 101 10 2 103 ...... 2 H 0.3 0.2 shRaptor 1 shRaptor 2 0.1 25 20 15 10 ** *** 5 0 0.0 shControl 1 2 shRaptor shRictor ...... 10% fetal bovine serum at 37C in 95% air/5% CO2 (Invitrogen, Carslbad, CA, USA). Small interfering ...
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Mikelis, Constantinos M., Zárate-Bladés, Carlos Rodrigo, Leelahavanichkul, Kantima, Wang, Zhiyong, Martin, Daniel, Doci, Colleen L., Dorsam, Robert T., Amornphimoltham, Panomwat, Gutkind, J. Silvio, Patel, Vyomesh, Weigert, Roberto, Masedunskas, Andrius, Molinolo, Alfredo A., and Dillenburg-Pilla, Patricia
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Multiple human malignancies rely on C-X-C motif chemokine receptor type 4 (CXCR4) and its ligand, SDF-1/CXCL12 (stroma cell-derived factor 1/C-X-C motif chemokine 12), to metastasize. CXCR4 inhibitors promote the mobilization...
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